| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays. |
| Immunogen | Synthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH. |
| Other Notes | To view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature. |
| Preparation Note | Purified by affinity chromatography on a column bearing the immunizing peptide. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Legal Information | FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |