F7425

Sigma

 

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:MFCD02262912

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Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
ApplicationThe antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
ImmunogenSynthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH.
Other NotesTo view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature.
Preparation NotePurified by affinity chromatography on a column bearing the immunizing peptide.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Legal InformationFLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody formaffinity isolated antibody
formbuffered aqueous solution
application(s)immunoblotting (chemiluminescent): 2.5 μg/mL using an Escherichia coli periplasmic extract expressing an N-terminal FLAGfusion protein
 indirect immunofluorescence: 5 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3